Unit+10-Gene+Expression

=**IMPORTANT NOTE--IF YOU WILL NOT BE HERE FRIDAY, YOU MUST TAKE THE EXAM THURSDAY 8th OR AFTER SCHOOL. IF YOU ARE MISSING THURSDAY AND FRIDAY, YOU MUST TAKE THE TEST ON MONDAY, MARCH 31 at 6:30 am!! LET ME KNOW YOUR PLAN**=

Thursday, March 20 Today we saw our bacteria GLOW!! We went over the answers to the lab questions for the transformation lab, microarray and paper plasmid lab. We reviewed operons and RNAi as well.

TO STUDY: Draw out and explain everything on paper: RNAi Microarrays Operons-repressible, inducible (both negative control) and the positive control of the Lac Operon How to make a recombinant plasmid How to transform bacteria Results of our lab--what happened and why

USE TARGETS AND WIKI!!

Wednesday, March 19 Today we did day 2 of the transformation lab. We first completed the worksheet below. For homework, work on the calculations and questions.

Read text section 20.1 - take notes related to Target I. Also, make sure tonight that you understand the lab that we are doing this week.
 * Tuesday, March 18**
 * Today we did day 1 of the Transformation lab.**
 * It is important that tonight you review for the test on Friday. It will not be an easy test, and will be VERY, VERY hard if you don't spend time with the material this week. Here are the targets again.**

Monday, March 17 Today we completed the paper plasmid lab. If you need help understanding genetic engineering, here is a clip: [|Genetic Engineering Clip]

We then discussed tomorrow's lab. If you were not here, you need to read the lab carefully and determine what the plates will look like. Here are some tips: The positive control should have lots of bacteria growing. The negative control should have no bacteria growing. The Starter Plate should have the word N/A in the column about whether it is positive or negative control. Here are some clips that may help: [|PGlo Lab] [|Transformation Lab] [|Making the plasmid and transformation]

Homework

Finish the questions from the paper plasmid lab Do the prelab for the transformation lab

Friday, March 14, 2014 Today we discussed the following two important topics: Positive Gene Regulation-At the end of this powerpoint: Eukaryotic Gene Regulation

Also, a bonus assignment is below. It is due the day after spring break. It deals with Epigenetics.

The homework is the Tools of the paper plasmid lab that is posted in the packet that was posted on Wednesday. Do the prelab (Part A) and cutting out the plasmid and gene and putting them together.

Thursday, March 13, 2014 Today we worked on the following worksheet. It says that it is a quiz, but it is not. Take your time and really think through the answers. Then, when you are all finished, go to the link on the page and check your answers. Homework is on the calendar. Check out what you need to read.

Wednesday, March 12, 2014 Today we learned about operons. You must spend time on this!! Here are resources: [|Lac Operon Animation 1] [|Lac Operon Animation 2]

[|Trp Operon Animation 1] [|Trp Operon Animation 2]

Tuesday, March 11, 2014 Today we did the microarray activity that is in the target packet. If you missed it, come get the materials from me. We also reviewed RNAi

Monday, March 10, 2014 Today we discussed the Genetic Disorders and Evolution Activity and watched a video about RNA i. Homework is the Virtual Mircoarray lab. Here are handouts from today in the packet below. Here is the link to the virtual lab: [|Virtual Microarray Lab]

Here is the link to the video: [|RNAi video]